Novel Molecular Networks and Regulatory MicroRNAs in Type 2 Diabetes Mellitus: Multiomics Integration and Interactomics Study

Background Type 2 diabetes mellitus (T2DM) is a metabolic disorder with severe comorbidities. A multiomics approach can facilitate the identification of novel therapeutic targets and biomarkers proper validation potential microRNA (miRNA) interactions. Objective The aim this study was to identify significant differentially expressed common target genes in various tissues their regulating miRNAs from publicly available Gene Expression Omnibus (GEO) data sets patients T2DM using silico analysis. Methods Using (DEGs) identified 5 sets, we performed functional enrichment, coexpression, network analyses pathways, protein-protein interactions, miRNA-mRNA interactions involved T2DM. Results We extracted 2852, 8631, 5501, 3662, 3753 DEGs expression profiles GEO GSE38642, GSE25724, GSE20966, GSE26887, GSE23343, respectively. DEG analysis showed that 16 were enriched insulin secretion, endocrine resistance, other T2DM-related pathways. Four DEGs, MAML3, EEF1D, NRG1, CDK5RAP2, important cluster regulated by commonly targeted (hsa-let-7b-5p, hsa-mir-155-5p, hsa-mir-124-3p, hsa-mir-1-3p), which are advanced glycation end products (AGE)-receptor for (RAGE) signaling pathway, culminating diabetic complications resistance. Conclusions This tissue-specific T2DM, especially pertaining heart, liver, pancreas. total top four targeting hsa-miR-124-3p, hsa-miR-1-3p, has-miR-155-5p). including phosphatidylinositol-3-kinase-protein kinase B, AGE-RAGE